The scaffold protein WRAP53beta orchestrates the ubiquitin response critical for DNA double-strand break repair.
Henriksson S, Rassoolzadeh H, Hedstrom E, Coucoravas C, Julner A, Goldstein M, Imreh G, Zhivotovsky B, Kastan MB, Helleday T, Farnebo M
Genes Dev. 2014 Dec 15;28(24):2726-38. doi: 10.1101/gad.246546.114. PubMed Article
Henriksson S, Rassoolzadeh H, Hedstrom E, Coucoravas C, Julner A, Goldstein M, Imreh G, Zhivotovsky B, Kastan MB, Helleday T, Farnebo M
Genes Dev. 2014 Dec 15;28(24):2726-38. doi: 10.1101/gad.246546.114. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 64671 | pEGFP-RNF8-N-terminus(1-111) | Expresses only N terminus of RNF8, amino acids 1 to 111 |
| 64672 | pEGFP-RNF8-Middle-part | Expresses only Middle part of RNF8, lacking N and C terminus, amino acids 112-401 |
| 64673 | pEGFP-RNF8-C-terminus | expresses only C terminus of RNF8, amino acids 402-485 |
| 64674 | p3xFLAG-CMV-RNF8-N-terminus(1-111) | Expresses only N terminus of RNF8, amino acids 1 to 111 |
| 64675 | p3xFLAG-CMV-RNF8-N-terminus(1-140) | expresses only N terminus of RNF8, amino acids 1 to 140 |
| 64676 | pEGFP-WRAP53beta | expresses Wild type WRAP53beta |
| 64677 | pEGFP-WRAP53beta-N-terminus | expresses only N terminal o WRAP53beta |
| 64678 | pEGFP-WRAP53beta-WD40domain | Expresses the WD40 domain of WRAP53beta |
| 64679 | pEGFP-WRAP53beta-C-terminus | expresses only C terminus of WRAP53beta |
| 64680 | pCMV-FLAG-WRAP53beta-siRNA-resistant | expresses siRNA resistant WRAP53beta |
| 64681 | pEGFP-WRAP53beta-DC-mutant-F164L | expresses single mutated WRAP53beta at F164L, found in Dyskeratosis congenita patients |
| 64682 | pEGFP-WRAP53beta-DC-mutant-H376Y | expresses single mutated WRAP53beta at H376Y, found in Dyskeratosis congenita patients |
| 64683 | pEGFP-WRAP53beta-DC-mutant-R398W | expresses single mutated WRAP53beta at R398W, found in Dyskeratosis congenita patients |
| 64684 | pEGFP-WRAP53beta-DC-mutant-G435R | expresses single mutated WRAP53beta at G435R, found in Dyskeratosis congenita patients |