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The scaffold protein WRAP53beta orchestrates the ubiquitin response critical for DNA double-strand break repair.
Henriksson S, Rassoolzadeh H, Hedstrom E, Coucoravas C, Julner A, Goldstein M, Imreh G, Zhivotovsky B, Kastan MB, Helleday T, Farnebo M
Genes Dev. 2014 Dec 15;28(24):2726-38. doi: 10.1101/gad.246546.114.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
64671pEGFP-RNF8-N-terminus(1-111)Expresses only N terminus of RNF8, amino acids 1 to 111
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64672pEGFP-RNF8-Middle-partExpresses only Middle part of RNF8, lacking N and C terminus, amino acids 112-401
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64673pEGFP-RNF8-C-terminusexpresses only C terminus of RNF8, amino acids 402-485
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64674p3xFLAG-CMV-RNF8-N-terminus(1-111)Expresses only N terminus of RNF8, amino acids 1 to 111
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64675p3xFLAG-CMV-RNF8-N-terminus(1-140)expresses only N terminus of RNF8, amino acids 1 to 140
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64676pEGFP-WRAP53betaexpresses Wild type WRAP53beta
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64677pEGFP-WRAP53beta-N-terminusexpresses only N terminal o WRAP53beta
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64678pEGFP-WRAP53beta-WD40domainExpresses the WD40 domain of WRAP53beta
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64679pEGFP-WRAP53beta-C-terminusexpresses only C terminus of WRAP53beta
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64680pCMV-FLAG-WRAP53beta-siRNA-resistantexpresses siRNA resistant WRAP53beta
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64681pEGFP-WRAP53beta-DC-mutant-F164Lexpresses single mutated WRAP53beta at F164L, found in Dyskeratosis congenita patients
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64682pEGFP-WRAP53beta-DC-mutant-H376Yexpresses single mutated WRAP53beta at H376Y, found in Dyskeratosis congenita patients
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64683pEGFP-WRAP53beta-DC-mutant-R398Wexpresses single mutated WRAP53beta at R398W, found in Dyskeratosis congenita patients
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64684pEGFP-WRAP53beta-DC-mutant-G435Rexpresses single mutated WRAP53beta at G435R, found in Dyskeratosis congenita patients
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