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One-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT).
Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB
BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5.
PubMed

Plasmids from Article

ID Plasmid Purpose  
62657pAYCMammalian expression of iCre recombinase from the broadly active CAG promoter/enhancer
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62658pBCWMammalian expression of PhiC31o recombinase from the broadly active CAG promoter/enhancer
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62659pBCVMammalian expression of FLPo recombinase from the broadly active CAG promoter/enhancer
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62660pBDTTargeting vector for PITT landing pad knock-in into the Rosa26 locus
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62661pBDUTargeting vector for PITT landing pad knock-in into the Rosa26 locus
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62662pBERDonor vector for tdTomato knock-in via landing pad
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62663pBDRDonor vector for tdTomato knock-in via landing pad
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62664pBGVDonor vector for mCFP knock-in via landing pad
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62665pBGWDonor vector for mCitrine knock-in via landing pad
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62666pBGXDonor vector for Dre knock-in via landing pad
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62667pBGTDonor vector for iRFP knock-in via landing pad
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62668pBFBDonor vector for tdTomato knock-in via landing pad
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62669pBEZDonor vector for tdTomato knock-in via landing pad
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62670pBBKVector for PhiC31o mRNA synthesis
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62671pBGODonor vector for mFTH1 knock-in via landing pad
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62672pBBJVector for FLPo mRNA synthesis
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