Optogenetic control of phosphoinositide metabolism.
Idevall-Hagren O, Dickson EJ, Hille B, Toomre DK, De Camilli P
Proc Natl Acad Sci U S A. 2012 Aug 28;109(35):E2316-23. doi: 10.1073/pnas.1211305109. Epub 2012 Jul 30.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 66836 | mCherry-CRY2-5ptaseOCRL | Expression of inositol 5-phosphatase domain of OCRL. Binds to CIBN upon blue light illumination. |
| 66837 | mCherry-CRY2-5ptaseOCRL(D523G) | Expression of inositol 5-phosphatase domain of OCRL(D523G - catalytically inactive mutant). Binds to CIBN upon blue light illumination. |
| 66839 | mCherry-CRY2-iSH2 | Expression of a light-inducible PI3-kinase, generated by fusing the iSH2 domain of human p85α (residues 420–615) to mCherry-CRY2. Binds endogenous PI3-kinase p110α catalytic subunits constitutively. |
| 66841 | iRFP-PH-PLCdelta1 | expression of near-infrared PI(4,5)P2 biosensor |
| 79561 | mCherry-CRY2-INPP5E | expression of mCherry-tagged CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motif |
| 79562 | CRY2-INPP5E | expression of CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motif |
| 79563 | mCherry-CRY2-INPP5E(D556A) | expression of mCherry-tagged CRY2PHR fused to catalytically dead INPP5E |
| 79564 | CRY2-INPP5E(D556A) | expression of CRY2PHR fused to catalytically dead INPP5E |
| 79565 | GFP-CRY2-OCRL | expression of GFP-tagged CRY2PHR fused to inositol 5-phosphatase domain of OCRL |
| 79567 | CRY2-OCRL(D523G) | expression of CRY2PHR fused to phosphatase dead mutant of OCRL |
| 79571 | CIBN-GFP-TMD | expression of CIBN domain fused to GFP tagged mouse Syntaxin1 TMD domain (transmembrane domain and polybasic region) |
| 79572 | Lyn11-CIBN-GFP | expression of plasma membrane signal from Lyn11 fused to CIBN and tagged with GFP |
| 79574 | CIBN-CAAX | expression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targeting |