Cumate-inducible gene expression system for sphingomonads and other Alphaproteobacteria.
Kaczmarczyk A, Vorholt JA, Francez-Charlot A
Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 10.1128/AEM.02296-13. Epub 2013 Aug 30.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 48094 | pQF-lacZ | Cumate-inducible expression of lacZ |
| 48095 | pQF | Cumate-inducible expression of 3xFLAG-tagged fusion protein |
| 48096 | pQH | Cumate-inducible expression of HA-tagged fusion protein |
| 48097 | pQM | Cumate-inducible expression of myc-tagged fusion protein |
| 48098 | pQY | Cumate-inducible expression of SYFP2-tagged fusion protein |
| 48099 | pQR | Cumate-inducible expression of mCherry-tagged fusion protein |
| 48100 | pQC | Cumate-inducible expression of ECFP-tagged fusion protein |
| 48101 | pQFD | Destination plasmid (Gateway cloning) for cumate-inducible expression of 3xFLAG-tagged fusion protein |
| 48102 | pQHD | Destination plasmid (Gateway cloning) for cumate-inducible expression of HA-tagged fusion protein |
| 48103 | pQMD | Destination plasmid (Gateway cloning) for cumate-inducible expression of myc-tagged fusion protein |
| 48104 | pQYD | Destination plasmid (Gateway cloning) for cumate-inducible expression of SYFP2-tagged fusion protein |
| 48107 | pAK501 | lacZ promoter-probe plasmid with pVS1 origin of replication and chloramphenicol resistance |