Plasma membrane restricted RhoGEF activity is sufficient for RhoA-mediated actin polymerization.
van Unen J, Reinhard NR, Yin T, Wu YI, Postma M, Gadella TW, Goedhart J
Sci Rep. 2015 Oct 5;5:14693. doi: 10.1038/srep14693.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 67894 | pmVenus-C2-MmMKL2 | Expresses a fusion of mVenus with MKL2 (MRTF-B) in mammalian cells |
| 67895 | p63(1-29)-mCherry-N1 | Expresses of fusion of mCherry with the N-terminal 29 amino acids of p63RhoGEF |
| 67896 | pmCherry-C1-p63RhoGEF | Expresses a fusion of mCherry with full-length p63RhoGEF in mammalian cells |
| 67897 | pmCherry-C1-pmDH | Expresses a fusion of mCherry with the membrane located DH domain (1-347) of p63RhoGEF in mammalian cells |
| 67898 | pmCherry-C1-DH | Expresses a fusion of mCherry with the DH domain (155-347) of p63RhoGEF in mammalian cells |
| 67899 | pYC3.60-C1 | Yellow Cameleon 3.60 in a clontech-style backbone for expression in mammalian cells |
| 67900 | pmCherry-FKBP12-C1 | Expresses a fusion of mCherry with FKBP12 in mammalian cells |
| 67901 | pmCherry-FKBP12-C1-DH | Expresses a fusion of mCherry with FKBP12 and the DH domain (155-347) of p63RhoGEF in mammalian cells |
| 67902 | Lck-FRB-ECFP(W66A) | Expresses a membrane located FRB domain in mammalian cells (the ECFP is not fluorescent) |
| 67903 | FRB-ECFP(W66A)-Giantin | Expresses a Golgi located FRB domain in mammalian cells (the ECFP is not fluorescent) |
| 67904 | ECFP(W66A)-FRB-MoA | Expresses a Mitochondria located FRB domain in mammalian cells (the ECFP is not fluorescent) |