CRISPR/Cas9 nuclease cleavage combined with Gibson assembly for seamless cloning.

Wang JW, Wang A, Li K, Wang B, Jin S, Reiser M, Lockey RF
Biotechniques. 2015 Apr 1;58(4):161-70. doi: 10.2144/000114261. eCollection 2015 Apr. PubMed Article

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