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Expression, purification, and characterization of authentic monoferric and apo-human serum transferrins.

Mason AB, Halbrooks PJ, Larouche JR, Briggs SK, Moffett ML, Ramsey JE, Connolly SA, Smith VC, MacGillivray RT
Protein Expr Purif. 2004 Aug;36(2):318-26. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
69601pNUT N6His Y95F/Y188F hTFNGExpresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the N-lobe
70084pNUTN6His Y426F/Y517F hTFNGExpresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the C-lobe
70085pNUT N6His Y95F/Y188F/Y426F/Y517F hTFNG hTFNGExpresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in either the N-lobe or the C-lobeN-lobe

Antibodies from Article