T. J. Kappock Lab: A multisubunit Gram-negative FGAM synthetase

Kappock
Unpublished

Plasmids from Article

ID	Plasmid	Purpose
72479	pJK520	Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); hypothetical protein with N-terminal His6 tag (H6AaOrfY), synthetic gene
72510	pJK522	Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6 tag (H6AaPurS); hypothetical protein with N-terminal His6 tag (H6AaOrfY), synthetic
72511	pJK530	Produces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster with C-terminal His6 tag (AaOrfYH6)
72512	pJK531	Produces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster (AaOrfY)
72513	pJK532	Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit with N-terminal His6 tag (H6AaPurQ)
72514	pJK533	Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)
72515	pJK539	Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit (AaPurL)
72516	pJK543	Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); large subunit (AaPurL)
72517	pJK544	Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)
72518	pJK546	Produces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster (AaOrfY)
72520	pJK549	Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit (AaPurL)
72521	pJK555	Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ)
72522	pJK567	Produces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster (AaOrfY)
72523	pJK598	Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with C-terminal His6 tag, and associated hypothetical protein (AaPurS, AaOrfY, AaPurQ, AaPurL)
72524	pJK599	Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, and associated hypothetical protein (AaPurS, AaOrfY, AaPurQ, AaPurLH6)
72526	pJK575	Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit with C-terminal His6 tag (AaPurLH6)
72527	pJK576	Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); hypothetical protein associated with purSyQL cluster (AaOrfY)
72528	pJK577	Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)
72529	pJK606	Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); glutaminase subunit (AaPurQ); large subunit with C-terminal His6 tag (AaPurLH6)
72530	pJK605	Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); glutaminase subunit (AaPurQ); large subuint (AaPurL)
72531	pJK610	Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ); large subunit wutg C-terminal His6 tag (AaPurLH6)
72532	pJK611	Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ); large subunit (AaPurL)
72533	pJK612	Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurL)
72534	pJK613	Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with His6 tag; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurLH6)
72535	pJK618	Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit, N-term His6 tag; glutaminase subunit; large subunit, C-terminal His6 tag (H6AaPurS, AaPurQ, AaPurLH6)
72536	pJK619	Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit, N-term His-tag; hypothet. protein; glutaminase subunit; large subunit (H6AaPurS, AaOrfY, AaPurQ, AaPurL)
72537	pJK620	Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6-tag (H6AaPurS)
72538	pJK622	Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit with N-terminal His6-tag (H6AaPurL)
72539	pJK624	Produces Acetobacter aceti 1023 OrfY; formylglycinamidine ribonucleotide synthetase glutaminase subunit; large subunit with C-terminal His6 tag (AaOrfY, AaPurQ, AaPurLH6)
72540	pJK623	Produces Acetobacter aceti 1023 purSyQL associated hypothetical protein; formylglycinamidine ribonucleotide synthetase glutaminase subunit; large subunit (AaOrfY, AaPurQ, AaPurL)
72541	pJK625	Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6 tag (H6AaPurS); glutaminase subunit (AaPurQ); large subunit (AaPurL)
74225	pJK545	Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ)
75379	pJK571	Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits and associated hypothetical protein with N-terminal His6 tag (AaPurS, H6AaOrfY, AaPurQ, AaPurL-G209D)