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SpyAvidin hubs enable precise and ultrastable orthogonal nanoassembly.
Fairhead M, Veggiani G, Lever M, Yan J, Mesner D, Robinson CV, Dushek O, van der Merwe PA, Howarth M
J Am Chem Soc. 2014 Sep 3;136(35):12355-63. doi: 10.1021/ja505584f. Epub 2014 Aug 21. PubMed Article

Plasmids from Article

ID Plasmid Purpose  
59547Dead Streptavidin-SpyCatcherpET21-DCatch encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyCatcher, for generation of chimeric SpyAvidin tetramers
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59548Dead Streptavidin-SpyTagpET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramers
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59549Traptavidin-E6pET21-Tre for expression of traptavidin (high stability mutant of streptavidin) bearing a glutamic acid tag for ion-exchange chromatography
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Recombinant Antibodies from Article