Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
A Comprehensive Toolbox for Genome Editing in Cultured Drosophila melanogaster Cells.
Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241. PubMed Article

Plasmids from Article

ID Plasmid Purpose
72851pSK23template for C-terminal eGFP tagging, Puromycin-resistance
72852pSK24template for C-terminal Twin-Strep tag, Puromycin-resistance
72853pSK25template for C-terminal double-FLAG tag, Puromycin-resistance
72854pSK32template for C-terminal TEV-site + V5-tag, Puromycin-Resistance
72855pSK15template for insertion of attP site (C-terminal tag plasmid design), Blasticidin-Resistance
72856pKF292template base vector for N-terminal tagging, inducible Blasticidin resistance
72857pKF293template for N-terminal 3x FLAG tag, inducible Blasticidin resistance
72858pKF294template for N-terminal 1x Strep tag, inducible Blasticidin resistance
72859pKF290template for N-terminal Strep-8xHis-Strep-TEV site tag, inducible Blasticidin resistance
72860pRB30template for N-terminal eGFP tag, inducible Blasticidin resistance
72861pRB36template base vector for N-terminal tagging, constitutive Blasticidin resistance
72862pRB34template for N-terminal 3x FLAG tag, constitutive Blasticidin resistance
72863pRB32template for N-terminal 1x Strep tag, constitutive Blasticidin resistance
72864pRB38template for N-terminal Strep-8xHis-Strep-TEV site tag, constitutive Blasticidin resistance
72865pRB40template for N-terminal eGFP tag, constitutive Blasticidin resistance
72866pRB35template base vector for N-terminal tagging, constitutive Puromycin resistance
72867pRB33template for N-terminal 3x FLAG tag, constitutive Puromycin resistance
72868pRB31template for N-terminal 1x Strep tag, constitutive Puromycin resistance
72869pRB37template for N-terminal Strep-8xHis-Strep-TEV site tag, constitutive Puromycin resistance
72870pRB39template for N-terminal eGFP tag, constitutive Puromycin resistance
72871pKF295expression of improved FLP recombinase
74773pKF296template for C-terminal TEV-site + 2x V5-tag (short version), Blasticidin-Resistance
74774pKF297template for C-terminal TEV-site + 2x V5-tag (short version), Puromycin-Resistance
74886pSK41template for insertion of attP site (C-terminal tag plasmid design), Puromycin-Resistance
74887pIS1targeting vector for C-terminal eGFP tag on Drosophila melanogaster Act5C

Antibodies from Article