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A comprehensive toolbox for genome editing in cultured Drosophila cells
Kunzelmann S, Böttcher R, Schmidts I, and Förstemann K.
G3 (Bethesda). Published online April 13,2016
Article

Plasmids from Article

ID Plasmid Purpose  
72851pSK23template for C-terminal eGFP tagging, Puromycin-resistance
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72852pSK24template for C-terminal Twin-Strep tag, Puromycin-resistance
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72853pSK25template for C-terminal double-FLAG tag, Puromycin-resistance
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72854pSK32template for C-terminal TEV-site + V5-tag, Puromycin-Resistance
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72855pSK15template for insertion of attP site (C-terminal tag plasmid design), Blasticidin-Resistance
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72856pKF292template base vector for N-terminal tagging, inducible Blasticidin resistance
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72857pKF293template for N-terminal 3x FLAG tag, inducible Blasticidin resistance
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72858pKF294template for N-terminal 1x Strep tag, inducible Blasticidin resistance
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72859pKF290template for N-terminal Strep-8xHis-Strep-TEV site tag, inducible Blasticidin resistance
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72860pRB30template for N-terminal eGFP tag, inducible Blasticidin resistance
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72861pRB36template base vector for N-terminal tagging, constitutive Blasticidin resistance
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72862pRB34template for N-terminal 3x FLAG tag, constitutive Blasticidin resistance
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72863pRB32template for N-terminal 1x Strep tag, constitutive Blasticidin resistance
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72864pRB38template for N-terminal Strep-8xHis-Strep-TEV site tag, constitutive Blasticidin resistance
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72865pRB40template for N-terminal eGFP tag, constitutive Blasticidin resistance
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72866pRB35template base vector for N-terminal tagging, constitutive Puromycin resistance
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72867pRB33template for N-terminal 3x FLAG tag, constitutive Puromycin resistance
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72868pRB31template for N-terminal 1x Strep tag, constitutive Puromycin resistance
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72869pRB37template for N-terminal Strep-8xHis-Strep-TEV site tag, constitutive Puromycin resistance
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72870pRB39template for N-terminal eGFP tag, constitutive Puromycin resistance
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72871pKF295expression of improved FLP recombinase
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74773pKF296template for C-terminal TEV-site + 2x V5-tag (short version), Blasticidin-Resistance
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74774pKF297template for C-terminal TEV-site + 2x V5-tag (short version), Puromycin-Resistance
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74886pSK41template for insertion of attP site (C-terminal tag plasmid design), Puromycin-Resistance
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74887pIS1targeting vector for C-terminal eGFP tag on Drosophila melanogaster Act5C
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