A comprehensive toolbox for genome editing in cultured Drosophila cells
Kunzelmann S, Böttcher R, Schmidts I, and Förstemann K.
G3 (Bethesda). Published online April 13,2016 Article
Kunzelmann S, Böttcher R, Schmidts I, and Förstemann K.
G3 (Bethesda). Published online April 13,2016 Article
Plasmids from Article
| ID | Plasmid | Purpose | |
|---|---|---|---|
| 72851 | pSK23 | template for C-terminal eGFP tagging, Puromycin-resistance |
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| 72852 | pSK24 | template for C-terminal Twin-Strep tag, Puromycin-resistance |
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| 72853 | pSK25 | template for C-terminal double-FLAG tag, Puromycin-resistance |
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| 72854 | pSK32 | template for C-terminal TEV-site + V5-tag, Puromycin-Resistance |
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| 72855 | pSK15 | template for insertion of attP site (C-terminal tag plasmid design), Blasticidin-Resistance |
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| 72856 | pKF292 | template base vector for N-terminal tagging, inducible Blasticidin resistance |
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| 72857 | pKF293 | template for N-terminal 3x FLAG tag, inducible Blasticidin resistance |
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| 72858 | pKF294 | template for N-terminal 1x Strep tag, inducible Blasticidin resistance |
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| 72859 | pKF290 | template for N-terminal Strep-8xHis-Strep-TEV site tag, inducible Blasticidin resistance |
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| 72860 | pRB30 | template for N-terminal eGFP tag, inducible Blasticidin resistance |
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| 72861 | pRB36 | template base vector for N-terminal tagging, constitutive Blasticidin resistance |
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| 72862 | pRB34 | template for N-terminal 3x FLAG tag, constitutive Blasticidin resistance |
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| 72863 | pRB32 | template for N-terminal 1x Strep tag, constitutive Blasticidin resistance |
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| 72864 | pRB38 | template for N-terminal Strep-8xHis-Strep-TEV site tag, constitutive Blasticidin resistance |
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| 72865 | pRB40 | template for N-terminal eGFP tag, constitutive Blasticidin resistance |
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| 72866 | pRB35 | template base vector for N-terminal tagging, constitutive Puromycin resistance |
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| 72867 | pRB33 | template for N-terminal 3x FLAG tag, constitutive Puromycin resistance |
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| 72868 | pRB31 | template for N-terminal 1x Strep tag, constitutive Puromycin resistance |
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| 72869 | pRB37 | template for N-terminal Strep-8xHis-Strep-TEV site tag, constitutive Puromycin resistance |
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| 72870 | pRB39 | template for N-terminal eGFP tag, constitutive Puromycin resistance |
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| 72871 | pKF295 | expression of improved FLP recombinase |
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| 74773 | pKF296 | template for C-terminal TEV-site + 2x V5-tag (short version), Blasticidin-Resistance |
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| 74774 | pKF297 | template for C-terminal TEV-site + 2x V5-tag (short version), Puromycin-Resistance |
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| 74886 | pSK41 | template for insertion of attP site (C-terminal tag plasmid design), Puromycin-Resistance |
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| 74887 | pIS1 | targeting vector for C-terminal eGFP tag on Drosophila melanogaster Act5C |
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