Multiple Gene Repression in Cyanobacteria Using CRISPRi.
Yao L, Cengic I, Anfelt J, Hudson EP
ACS Synth Biol. 2015 Dec 28.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 73220 | pMD19T-psba1-Ppsba2-dCas9-SpR | Contains dCas9 from S. pyogenes under constitutive promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation. |
| 73221 | pMD19T-slr0230-PL31-sgRNANT1-KmR | Contains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli |
| 73223 | pMD19T-psba1-TetR-PL22-dCas9-SpR | Contains dCas9 from S. pyogenes under aTc inducible promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation. |
| 73224 | pMD19T-slr0230-PL22-sgRNANT1-KmR | Contains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli |