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The nuclear basket mediates perinuclear mRNA scanning in budding yeast.
Saroufim MA, Bensidoun P, Raymond P, Rahman S, Krause MR, Oeffinger M, Zenklusen D
J Cell Biol. 2015 Dec 21;211(6):1131-40. doi: 10.1083/jcb.201503070.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
72230pDZ514 pURA MET25p PP7-PS-2x-yeGFPUracil selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoter
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72233pDZ529 pTRP MET25p PP7-PS-2x-yeGFPTryptophan selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoter
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72234pDZ536 pURA ADE3p PP7-PS-2x-yeGFPUracil selection; Expression of PP7-2x-yeGFP fusion protein under the ADE3 promoter
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72236pDZ585 pKAN 2x-mCherryFloxed Kanamycin (G418) selection maker; 3' tagging vector used to create C-terminal fusion proteins with 2x-mCherry
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72237pDZ617 pKAN 12xPP7 V4Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 12 repetitive PP7 version 4 RNA stem-loops to gene of interest
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72240pDZ305 GTL1 GAL1p 24xPP7 V3 <HIS>Histidine selection; Used to insert a GAL1 promoter and 24 repetitive PP7 version 3 RNA stem-loops before GLT1's start codon (5' tagging)
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73172pDZ584 pKAN 1x-mCherryFloxed Kanamycin (G418) selection maker; 3' tagging vector used to create C-terminal fusion proteins with 1x-mCherry
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73173pDZ645 pKAN 1x-mCherry-12xPP7 V4Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 1x-mCherry followed by 12 repetitive PP7 version 4 RNA stem-loops to gene of interest
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