A Tunable Protein Piston That Breaks Membranes to Release Encapsulated Cargo.
Polka JK, Silver PA
ACS Synth Biol. 2016 Feb 3.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 71224 | Reb_1 aka PAS619 | Expresses wild type R bodies from C. taeniospiralis in pETM11 backbone |
| 71225 | Reb_79 aka PAS620 | Reb_1 with RebA(Q91R) |
| 71226 | Reb_80 aka PAS621 | Reb_1 with RebB(I67T, N84I) |
| 71227 | Reb_81 aka PAS622 | Reb_1 with RebB(T28A, D72G) |
| 71228 | Reb_82 aka PAS623 | Reb_1 with RebB(S82P, D88G) |
| 71229 | Reb_83 aka PAS624 | Reb_1 with RebA(G53A, D89G) |
| 71230 | Reb_140 aka PAS625 | Reb_1 with RebA(S88P) |
| 71231 | Reb_65 aka PAS626 | Reb_1 with RebA(Y82A) |
| 71232 | Reb_67 aka PAS627 | Reb_1 with RebA(S88A) |
| 71233 | Reb_221 aka PAS628 | Reb_1 with RebA(G90A) |
| 71234 | Reb_206 aka PAS629 | Expresses wild type R bodies with mNeonGreen-tagged second copy of RebB under a weak RBS downstream as well as soluble mCherry |
| 71235 | Reb_207 aka PAS630 | Reb_206 with RebA(S88P) |