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Versatile vectors for efficient mutagenesis of Bradyrhizobium diazoefficiens and other Alphaproteobacteria.

Ledermann R, Strebel S, Kampik C, Fischer HM
Appl Environ Microbiol. 2016 Feb 26. pii: AEM.04085-15. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
74103pREDSIXmCherry-encoding vector for marker replacement mutagenesis in Bradyrhizobium diazoefficiens and other Alphaproteobacteria
74104pTETSIXmCherry-encoding vector for markerless deletion mutagenesis in Bradyrhizobium diazoefficiens and other Alphaproteobacteria
74106pRGD-GmRPlasmid containing gentamicin resistance gene cassette flanked by multiple restriction sites
74107pRGD-HmRPlasmid containing hygromycin B resistance gene cassette flanked by multiple restriction sites
74108pRGD-KmRPlasmid containing kanamycin resistance gene cassette flanked by multiple restriction sites
74109pRGD-SmRPlasmid containing streptomycin resistance gene cassette flanked by multiple restriction sites
74110pRGD-TcRPlasmid containing tetracycline resistance gene cassette flanked by multiple restriction sites

Antibodies from Article