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Molecular architecture of 4E-BP translational inhibitors bound to eIF4E.

Peter D, Igreja C, Weber R, Wohlbold L, Weiler C, Ebertsch L, Weichenrieder O, Izaurralde E
Mol Cell. 2015 Mar 19;57(6):1074-87. doi: 10.1016/j.molcel.2015.01.017. Epub 2015 Feb 19. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
79437pT7-EGFP-HseIF4EExpresses EGFP-tagged HseIF4E in Mammalian cells
79438pLNHA-C1-Hs4E-BP1Expresses lambdaN-HA-tagged human 4E-BP1 in Mammalian cells
79439pLNHA-C1-Hs4E-BP3Expresses lambdaN-HA-tagged human 4E-BP3 in Mammalian cells
148256pnEA-NpM-Dm4E-T_9-44-F41DW42D-GB1_AABacterial Expression of Dm4E-T_9-44-F41DW42D
148257pnEA-NpM-Dm4E-T_9-44-K12E-GB1_AABacterial Expression of Dm4E-T_9-44-K12E
148258pnEA-NpM-Dm4E-T_9-44-K12ER19E-GB1_AABacterial Expression of Dm4E-T_9-44-K12ER19E
148259pnEA-NpM-Dm4E-T_9-44-K12ER19EK43E-GB1_AABacterial Expression of Dm4E-T_9-44-K12ER19EK43E
148260pnEA-NpM-Dm4E-T_9-44-R19E-GB1_AABacterial Expression of Dm4E-T_9-44-R19E
148261pnEA-NpM-Dm4E-T_9-44-YLFWtoAADD-GB1_AABacterial Expression of Dm4E-T_9-44-YLFWtoAADD
148262pnEA-NpM-Dmchim4EBP-GB1_AABacterial Expression of Dmchim4EBP
148263pnEA-NpM-DmCUP_325-376-R329E-GB1_AABacterial Expression of DmCup_325-376-R329E
148264pnEA-NpM-DmCUP_325-376-R329ER336E-GB1_AABacterial Expression of DmCup_325-376-R329ER336E
148265pnEA-NpM-DmCUP_325-376-R329ER336ER375E-GB1_AABacterial Expression of DmCup_325-376-R329ER336ER375E
148266pnEA-NpM-DmCUP_325-376-R336E-GB1_AABacterial Expression of DmCup_325-376-R336E
148267pnEA-NpM-DmThor_32-83-GB1_AABacterial Expression of DmThor_32-83
148268pnEA-NpM-DmThor_32-83-S65DT70D-GB1_AABacterial Expression of DmThor_32-83-S65DT70D
148269pnEA-NpM-DmThor_32-83-YMSTtoAADD-GB1_AABacterial Expression of DmThor_32-83-YMSTtoAADD
148270pnEA-NpM-DmThor_50-83-R56AR63A-GB1_AABacterial Expression of DmThor_50-83-R56ER63A
148271pnEA-NpM-DmThor_50-83-R56E-GB1_AABacterial Expression of DmThor_50-83-R56E
148272pnEA-NpM-DmThor_50-83-R56ER63E-GB1_AABacterial Expression of DmThor_50-83-R56ER63E
148273pnEA-NpM-DmThor_50-83-R63E-GB1_AABacterial Expression of DmThor_50-83-R63E
148274pnEA-NpM-DmThor_50-83-YMVLtoAADD-GB1_AABacterial Expression of DmThor_50-83-YMVLtoAADD
148275pnEA-NpM-Hs4EBP1_50-83-GB1_AABacterial Expression of Hs4EBP1_50-83
148276pnEA-NpM-Hs4EBP1_50-83-L75AV81A-GB1_AABacterial Expression of Hs4EBP1_50-83-L75AV81A
148277pnEA-NpM-Hs4EBP1_50-83-Y54AL59A-GB1_AABacterial Expression of Hs4EBP1_50-83-Y54AL59A
148278pnEA-NpM-Hs4EBP1_50-83-YLLVtoAAAA-GB1_AABacterial Expression of Hs4EBP1_50-83-YLLVtoAAAA
148279pnEA-NpM-Hs4EBP3_36-67-GB1_AABacterial Expression of Hs4EBP3_36-67
148280pnEA-NpM-Hs4EBP3_36-69-Y40AL45A-GB1_AABacterial Expression of Hs4EBP3_36-69-Y40AL45A
148281pnEA-NpM-Hs4EBP3_36-69-YLLVtoAAA-GB1_AABacterial Expression of Hs4EBP3_36-69-YLLVtoAAAA
148282pnYC-NpH-HseIF4E_36-217_AABacterial Expression of HseIF4E_36-217
148372pAc5.1B-EGFP-Dm4E-T-F41DW42D_ABInsect Expression of Dm4E-T-F41DW42D
148373pAc5.1B-EGFP-Dm4E-T-YLFWtoAADD_ABInsect Expression of Dm4E-T-YLFWtoAADD
148374pAc5.1B-EGFP-Dmchim4EBP_ABInsect Expression of Dmchim4EBP
148375pAc5.1B-EGFP-DmThor-S65DT70D_ABInsect Expression of DmThor-S65DT70D
148376pAc5.1B-lambdaN-HA-DmThor_50-83-YMVLtoAADD_ABInsect Expression of DmThor_50-83-YMVLtoAADD
148377pAc5.1B-lambdaN-HA-DmThor-V75DL79D_ABInsect Expression of DmThor-V75DL79D
148378pnEA-NpM-DmThor_50-83-R56ER63ER81E-GB1_ABBacterial Expression of DmThor_50-83-R56ER63ER81E
148379pnEA-NpM-DmThor_50-83-V75DL79D-GB1_ABBacterial Expression of DmThor_50-83-V75DL79D
148380pnEA-NpM-Hs4EBP3_36-69-L61A V67A-GB1_ABBacterial Expression of Hs4EBP3_36-69-L61AV67A

Antibodies from Article