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pLNHA-C1-Hs4E-BP3
(Plasmid #79439)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79439 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 79439-D50 50 μg of DNA in Tris buffer $495
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DNA 79439-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pLNHA-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4138
  • Total vector size (bp) 4177
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hs4E-BP3
  • Alt name
    EIF4EBP3
  • Alt name
    eukaryotic initiation factor 4E-binding protein 3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    303
  • GenBank ID
    NM_003732
  • Entrez Gene
    EIF4EBP3 (a.k.a. 4E-BP3, 4EBP3)
  • Promoter CMV
  • Tag / Fusion Protein
    • lambdaN-HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-forw
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLNHA-C1-Hs4E-BP3 was a gift from Elisa Izaurralde (Addgene plasmid # 79439 ; http://n2t.net/addgene:79439 ; RRID:Addgene_79439)

  • For your References section:

    Molecular architecture of 4E-BP translational inhibitors bound to eIF4E. Peter D, Igreja C, Weber R, Wohlbold L, Weiler C, Ebertsch L, Weichenrieder O, Izaurralde E. Mol Cell. 2015 Mar 19;57(6):1074-87. doi: 10.1016/j.molcel.2015.01.017. Epub 2015 Feb 19. 10.1016/j.molcel.2015.01.017 PubMed 25702871
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