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A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes.
Sidik SM, Huet D, Ganesan SM, Huynh MH, Wang T, Nasamu AS, Thiru P, Saeij JP, Carruthers VB, Niles JC, Lourido S
Cell. 2016 Sep 8;166(6):1423-1435.e12. doi: 10.1016/j.cell.2016.08.019. Epub 2016 Sep 2.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
80322pU6-SAG1-DHFREncodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamine
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80323pCas9-CATEncodes Cas9 and chloramphenicol acetyltransferase (CAT)
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80324pU6-DecoyEncodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondii
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80329pU6-DHFRAllows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamine
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80636Lourido Toxoplasma CRISPR Library V1Plasmid sgRNA library containing ten guides against each of the 8,158 predicted Toxoplasma gondii protein coding genes.
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