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pCas9-CAT
(Plasmid #80323)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 80323 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pU6-Universal
  • Backbone size w/o insert (bp) 8360
  • Total vector size (bp) 9301
  • Modifications to backbone
    The sgRNA region has been replaced with chloramphenicol acetyltransferase with the TUB1 5'UTR and the SAG1 3' UTR
  • Vector type
    CRISPR
  • Selectable markers
    CAT

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Cas9
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    4101
  • Promoter TgTUB1
  • Tags / Fusion Proteins
    • 3X FLAG (N terminal on insert)
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Chloramphenicol acetyltransferase
  • Alt name
    CAT
  • Insert Size (bp)
    1535
  • Promoter TgTUB1

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site PciI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer pBluescript-KS
  • 3′ sequencing primer pBluescript-SK
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCas9-CAT was a gift from Sebastian Lourido (Addgene plasmid # 80323 ; http://n2t.net/addgene:80323 ; RRID:Addgene_80323)
  • For your References section:

    A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik SM, Huet D, Ganesan SM, Huynh MH, Wang T, Nasamu AS, Thiru P, Saeij JP, Carruthers VB, Niles JC, Lourido S. Cell. 2016 Sep 8;166(6):1423-1435.e12. doi: 10.1016/j.cell.2016.08.019. Epub 2016 Sep 2. 10.1016/j.cell.2016.08.019 PubMed 27594426