Tools and strategies for scarless allele replacement in Drosophila using CRISPR/Cas9.
Lamb AM, Walker EA, Wittkopp PJ
Fly (Austin). 2016 Aug 5:1-12.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 83473 | pGEM-WingGFP-tan | EGFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice. |
| 83474 | pGEM-3XP3.RFP-tan | RFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice. |