Sumoylation of Rap1 mediates the recruitment of TFIID to promote transcription of ribosomal protein genes.
Chymkowitch P, Nguea AP, Aanes H, Koehler CJ, Thiede B, Lorenz S, Meza-Zepeda LA, Klungland A, Enserink JM
Genome Res. 2015 Jun;25(6):897-906. doi: 10.1101/gr.185793.114. Epub 2015 Mar 23.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 84525 | pDONR221-RAP1 | This plasmid can be used as a donor for subcloning into Gateway destination vectors. |
| 84527 | pDONR221-rap1-K9R | This plasmid can be used as a donor for subcloning into Gateway destination vectors. |
| 84528 | pDONR221-rap1-K651R | This plasmid can be used as a donor for subcloning into Gateway destination vectors. |
| 84531 | pAG415-ADH1p-HA-rap1-K9R | This plasmid can be used to express HA-tagged Rap1-K9R from the ADH promoter in yeast |
| 84533 | pAG415-GPDp-EGFP-rap1-K3NR | This plasmid can be used to express EGFP-tagged Rap1K3NR from the GDP promoter in yeast |
| 84534 | pAG415-GPDp-EGFP-rap1-K3CR | This plasmid can be used to express EGFP-tagged Rap1K3CR from the GDP promoter in yeast |
| 84535 | pAG415-GPDp-EGFP-rap1-K9R | This plasmid can be used to express EGFP-tagged Rap1K9R from the GDP promoter in yeast |
| 84536 | pAG415-GPDp-EGFP-rap1-K651R | This plasmid can be used to express EGFP-tagged Rap1-K651R from the GDP promoter in yeast |
| 84564 | pDONR221-rap1-K462-463-651R | This plasmid can be used as a donor for subcloning into Gateway destination vectors. |
| 84565 | pDONR221-rap1-K43-117-118R | This plasmid can be used as a donor for subcloning into Gateway destination vectors. |