Electroporation of Cas9 protein/sgRNA into early pronuclear zygotes generates non-mosaic mutants in the mouse.
Hashimoto M, Yamashita Y, Takemoto T
Dev Biol. 2016 Oct 1;418(1):1-9. doi: 10.1016/j.ydbio.2016.07.017. Epub 2016 Jul 26.
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