CPI motif interaction is necessary for capping protein function in cells.
Edwards M, McConnell P, Schafer DA, Cooper JA
Nat Commun. 2015 Sep 28;6:8415. doi: 10.1038/ncomms9415.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 89245 | pBJ 2358 | Expresses YFP fused to the N-terminus of Mouse Capping Protein Alpha1, using the Clontech vector PEYFP C-1. |
| 253114 | CPAlpha1Beta2(R15A,Y79A) pBJ2304 | pBJ2304 Cooper Lab. Bacterial expression of His-tagged mouse CP heterodimer (alpha1, beta2 isoforms). Two point mutations of the beta2 subunit (R15A, Y79A). Little to no binding of CPI motifs. |
| 253187 | pBJ2363 GFP mouse capping protein beta2 (R15A,Y79A) | Expresses GFP fusion of mouse capping protein beta2 subunit with two point mutations that inactivate binding to CPI motifs. |