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A Modular Toolkit for Generating Pichia pastoris Secretion Libraries.
Obst U, Lu TK, Sieber V
ACS Synth Biol. 2017 Mar 15. doi: 10.1021/acssynbio.6b00337.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
84960pPTK001-2-pAOX1Type 2, pAOX1, Alcohol oxidase 1 promoter
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84961pPTK002-2-pGAPType 2, pGAP, Glyceraldehyde-3-phosphate dehydrogenase promoter
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84962pPTK003-2-pENO1Type 2, pENO1, Enolase 1 promoter
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84963pPTK004-2-pTPI1Type 2, pTPI1, Triose phosphate isomerase 1 promoter
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84964pPTK005-3a-αMFType 3a, α-mating factor - secretion signal
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84965pPTK006-3a-αMF no EAEAType 3a, α-mating factor no EAEA - secretion signal
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84966pPTK007-3a-αMFΔType 3a, α-mating factor pre-sequence shortened - secretion signal
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84967pPTK008-3a-αMFΔ no KexType 3a, α-mating factor no Kex recognition site - secretion signal
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84968pPTK009-3a-αAmylase-αMFΔType 3a, α-Amylase followed by αMFΔ - secretion signal
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84969pPTK010-3a-Glucoamylase-αMFΔType 3a, Glucoamylase followed by αMFΔ - secretion signal
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84970pPTK011-3a-SA-αMFΔType 3a, Serum albumin followed by αMFΔ - secretion signal
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84971pPTK012-3a-Inulinase-αMFΔType 3a, Inulinase followed by αMFΔ - secretion signal
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84972pPTK013-3a-Invertase-αMFΔType 3a, Invertase followed by αMFΔ - secretion signal
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84973pPTK014-3a-Killer-αMFΔType 3a, Killer followed by αMFΔ - secretion signal
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84974pPTK015-3-yEGFP_IntraType 3, Green fluorescent protein
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84975pPTK016-3-RFP_IntraType 3, Red fluorescent protein
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84976pPTK017-3b-yEGFP_SecType 3b, Green fluorescent protein
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84977pPTK018-3b-RFP_SecType 3b, Red fluorescent protein
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84988pPTK019-4-tAOX1Type 4, tAOX, Alcohol oxidase 1 terminator
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84989pPTK020-7-attBType 7, BxbI recognition site
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84990pPTK021-7-PARSType 7, Pichia autonomously replicating sequence
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