A Modular Toolkit for Generating Pichia pastoris Secretion Libraries.
Obst U, Lu TK, Sieber V
ACS Synth Biol. 2017 Mar 15. doi: 10.1021/acssynbio.6b00337. PubMed Article
Obst U, Lu TK, Sieber V
ACS Synth Biol. 2017 Mar 15. doi: 10.1021/acssynbio.6b00337. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 84960 | pPTK001-2-pAOX1 | Type 2, pAOX1, Alcohol oxidase 1 promoter |
| 84961 | pPTK002-2-pGAP | Type 2, pGAP, Glyceraldehyde-3-phosphate dehydrogenase promoter |
| 84962 | pPTK003-2-pENO1 | Type 2, pENO1, Enolase 1 promoter |
| 84963 | pPTK004-2-pTPI1 | Type 2, pTPI1, Triose phosphate isomerase 1 promoter |
| 84964 | pPTK005-3a-αMF | Type 3a, α-mating factor - secretion signal |
| 84965 | pPTK006-3a-αMF no EAEA | Type 3a, α-mating factor no EAEA - secretion signal |
| 84966 | pPTK007-3a-αMFΔ | Type 3a, α-mating factor pre-sequence shortened - secretion signal |
| 84967 | pPTK008-3a-αMFΔ no Kex | Type 3a, α-mating factor no Kex recognition site - secretion signal |
| 84968 | pPTK009-3a-αAmylase-αMFΔ | Type 3a, α-Amylase followed by αMFΔ - secretion signal |
| 84969 | pPTK010-3a-Glucoamylase-αMFΔ | Type 3a, Glucoamylase followed by αMFΔ - secretion signal |
| 84970 | pPTK011-3a-SA-αMFΔ | Type 3a, Serum albumin followed by αMFΔ - secretion signal |
| 84971 | pPTK012-3a-Inulinase-αMFΔ | Type 3a, Inulinase followed by αMFΔ - secretion signal |
| 84972 | pPTK013-3a-Invertase-αMFΔ | Type 3a, Invertase followed by αMFΔ - secretion signal |
| 84973 | pPTK014-3a-Killer-αMFΔ | Type 3a, Killer followed by αMFΔ - secretion signal |
| 84974 | pPTK015-3-yEGFP_Intra | Type 3, Green fluorescent protein |
| 84975 | pPTK016-3-RFP_Intra | Type 3, Red fluorescent protein |
| 84976 | pPTK017-3b-yEGFP_Sec | Type 3b, Green fluorescent protein |
| 84977 | pPTK018-3b-RFP_Sec | Type 3b, Red fluorescent protein |
| 84988 | pPTK019-4-tAOX1 | Type 4, tAOX, Alcohol oxidase 1 terminator |
| 84989 | pPTK020-7-attB | Type 7, BxbI recognition site |
| 84990 | pPTK021-7-PARS | Type 7, Pichia autonomously replicating sequence |