The crystal structure of S. cerevisiae Sad1, a catalytically inactive deubiquitinase that is broadly required for pre-mRNA splicing.

Hadjivassiliou H, Rosenberg OS, Guthrie C
RNA. 2014 May;20(5):656-69. doi: 10.1261/rna.042838.113. Epub 2014 Mar 28. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
90297	pET28b-Sad1	protein expression
90299	pET28b-ZnF-UBPdel	protein expression
90302	pET28b-sad1-C63A	protein expression
90303	pET28b-sad1-H79A	protein expression
90304	pET28b-sad1-H85A	protein expression
90305	pET28b-sad1-D159A	protein expression
90306	pET28b-sad1-H405A	protein expression
90307	pET28b-sad1-D422A	protein expression
90308	pRS313-Sad1-Wt	yeast expression