TRIM28 regulates the nuclear accumulation and toxicity of both alpha-synuclein and tau.
Rousseaux MW, de Haro M, Lasagna-Reeves CA, De Maio A, Park J, Jafar-Nejad P, Al-Ramahi I, Sharma A, See L, Lu N, Vilanova-Velez L, Klisch TJ, Westbrook TF, Troncoso JC, Botas J, Zoghbi HY
Elife. 2016 Oct 25;5. pii: e19809. doi: 10.7554/eLife.19809.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 92194 | pLenti-DsRed_IRES_EGFP | Lentiviral overexpression vector to make stable bicistronic cell line for control (EGFP) screen |
| 92195 | pLenti-DsRed_IRES_SNCA:EGFP | Lentiviral overexpression vector to make stable bicistronic cell line for α-Syn screen |
| 92196 | pLenti-DsRed_IRES_MAPT:EGFP | Lentiviral overexpression vector to make stable bicistronic cell line for Tau screen |
| 92199 | pKH3-TRIM28-C65A/C68A | Mammalian overexpression vector for TRIM28; catalytically inactive (mutated on C65A and C68A) |
| 92200 | pINDUCER20-α-Syn | Retroviral overexpression vector (doxycycline-inducible) for α-Syn |
| 92201 | pINDUCER20-Tau | Retroviral overexpression vector (doxycycline-inducible) for Tau |
| 92203 | pBABE-α-Syn-nYFP | Retroviral overexpression vector for α-Syn bimolecular fluorescence complementation (BiFC) |
| 92204 | pBABE-Tau-nYFP | Retroviral overexpression vector for Tau bimolecular fluorescence complementation (BiFC) |
| 92205 | pBABE-Tau-cYFP | Retroviral overexpression vector for α-Syn/Tau bimolecular fluorescence complementation (BiFC) |