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Long-term self-renewing human epicardial cells generated from pluripotent stem cells under defined xeno-free conditions.
Bao X, Lian X, Hacker TA, Schmuck EG, Qian T, Bhute VJ, Han T, Shi M, Drowley L, Plowright A, Wang QD, Goumans MJ, Palecek SP
Nat Biomed Eng. 2016;1. pii: 0003. doi: 10.1038/s41551-016-0003. Epub 2016 Dec 5.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
82333WT1-2A-eGFP PGK PuroR Donor PlasmidWT1 targeting donor
92312WT1 KI gRNA1CRISPR-GFP-gRNA for cutting WT1
92313WT1 KI gRNA2CRISPR-GFP-gRNA for cutting WT1

Antibodies from Article