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Nontransgenic Marker-Free Gene Disruption by an Episomal CRISPR System in the Oleaginous Microalga, Nannochloropsis oceanica CCMP1779.
Poliner E, Takeuchi T, Du ZY, Benning C, Farre EM
ACS Synth Biol. 2018 Apr 20;7(4):962-968. doi: 10.1021/acssynbio.7b00362. Epub 2018 Mar 14.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
98147pNOC-CRISPR-P2A-BlastRExpresses Cas9-Nlux-P2A-BlastR and sgRNA in Nannochloropsis oceanica, and CEN/ARS region for episomal expression.
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99369pNOC-CRISPR-compactExpresses Cas9-Nlux and sgRNA. Contains CEN/ARS. No selection marker.
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99370pNOC-CRISPR-HAExpresses Cas9-HA and sgRNA. Hygromycin resistance.
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99861pNOC-CRISPR-35SExpresses Cas9-Nlux and sgRNA. Contains CEN/ARS and 35 terminator. No selection marker.
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99863pNOC-ARS-CRISPR-v2Expresses Cas9-Nlux and sgRNA. Compact backbone contains CEN/ARS. Hygromycin selection marker.
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100008pNOC-CRISPRExpresses Cas9-Nlux and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into genome
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100009pNOC-CRISPR-GFPExpresses Cas9-GFP and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into geneome
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101009pNSA-ARS-CRISPR-BlastRExpresses Cas9-Nlux and sgRNA and contains CEN/ARS. CCMP537 LDSP promoter driven Blasticidin resistance gene.
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Recombinant Antibodies from Article