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pNOC-CRISPR-compact
(Plasmid #99369)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 99369 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pNOC-ARS-CRISPR-compact
  • Backbone size (bp) 10166
  • Vector type
    Algae, Nannochloropsis expression
  • Promoter Ribi promoter
  • Tag / Fusion Protein
    • Cas9-Nlux

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer ACAAGATAAAAGGTAGTATTTGTTGGC
  • 3′ sequencing primer ACTTCCTGCTCTCAGGTATTAATG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNOC-CRISPR-compact was a gift from Eva Farre (Addgene plasmid # 99369 ; http://n2t.net/addgene:99369 ; RRID:Addgene_99369)
  • For your References section:

    Nontransgenic Marker-Free Gene Disruption by an Episomal CRISPR System in the Oleaginous Microalga, Nannochloropsis oceanica CCMP1779. Poliner E, Takeuchi T, Du ZY, Benning C, Farre EM. ACS Synth Biol. 2018 Apr 20;7(4):962-968. doi: 10.1021/acssynbio.7b00362. Epub 2018 Mar 14. 10.1021/acssynbio.7b00362 PubMed 29518315