Reversible Optogenetic Control of Subcellular Protein Localization in a Live Vertebrate Embryo.
Buckley CE, Moore RE, Reade A, Goldberg AR, Weiner OD, Clarke JD
Dev Cell. 2016 Jan 11;36(1):117-26. doi: 10.1016/j.devcel.2015.12.011. PubMed Article
Buckley CE, Moore RE, Reade A, Goldberg AR, Weiner OD, Clarke JD
Dev Cell. 2016 Jan 11;36(1):117-26. doi: 10.1016/j.devcel.2015.12.011. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 154910 | pCS2+-N-PAS2-GAF-PHYB-MCherry-CAAX | 1-621 amino acids of Arabidopsis PHYB (Gene ID: 816394), tagged with mCherry fluorescence protein and the CAAX membrane moiety. Mammalian codon optimised. |
| 154911 | pCS2+-Y276H PHYB-CAAX | 1-621 amino acids of Arabidopsis PHYB (as in construct Addgene ID 154910) with the CAAX membrane moiety tag. Tyrosine residue 276 of PHYB was mutated to Histidine. |
| 154913 | pCS2+-PIF6-EGFP | 1-100 amino acids of Arabidopsis PIF6 (Gene ID: 825382), tagged with EGFP fluorescent protein. Mammalian codon optimised. |
| 154914 | pCS2+-Pard3-EGFP-PIF6 | Full length zebrafish Pard3 (Gene ID: 403050), tagged with EGFP fluorescent protein and Arabidopsis PIF6 (as in construct Addgene ID 154913). |