FnCpf1: a novel and efficient genome editing tool for Saccharomyces cerevisiae.
Swiat MA, Dashko S, den Ridder M, Wijsman M, van der Oost J, Daran JM, Daran-Lapujade P
Nucleic Acids Res. 2017 Dec 1;45(21):12585-12598. doi: 10.1093/nar/gkx1007. PubMed Article
Swiat MA, Dashko S, den Ridder M, Wijsman M, van der Oost J, Daran JM, Daran-Lapujade P
Nucleic Acids Res. 2017 Dec 1;45(21):12585-12598. doi: 10.1093/nar/gkx1007. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 103008 | pUDE731 | S. cerevisiae Episomal plasmid harboring Fncpf1 under the control of TDH3p |
| 103018 | pUD628 | expression of a Cpf1 programming crRNA targetting ADE2 (crADE2-3.S) |
| 103019 | pUDC175 | S. cerevisae centromic plasmid harboring Fncpf1 under control of TEF1 promoter |
| 103020 | pUDE710 | expression of a Cpf1 programming crRNA targeting ADE2 and HIS4 (crADE2-3 crHIS4-4.S) |
| 103021 | pUDE714 | expression of a Cpf1 programming crRNA targetting HIS4 (crHIS4-4.S) |
| 103022 | pUDE722 | expression of a Cpf1 programming crRNA targetting CAN1 (crCAN1-4S) |
| 103023 | pUDE724 | expression of a Cpf1 programming crRNA targetting PDR12 (crPDR12-3S) |
| 103024 | pUDE735 | expression of a Cpf1 programming crRNA targetting CAN1, HIS4, PDR12 and ADE2 (crCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S) |