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A modular toolbox for Golden-Gate-based plasmid assembly streamlines generation of Ralstonia solanacearum species complex knockout strains and multi-cassette complementation constructs.

Wu D, Schandry N, Lahaye T
Mol Plant Pathol. 2017 Oct 27. doi: 10.1111/mpp.12632. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
104514LI A-B phrpB LI promoter module
104515LI A-B pEPS LI promoter module
104516LI A-B pGala7 LI promoter module
104517LI C-D GUSLI Gene of Interest module
104518LI C-D DestLI Gateway cassette
104519LI D-E V5 LI C-terminal module
104520LI E-F TfdLI terminator module
104521LI F-G SpecLI resistance gene module
104522LI F-G GentLI resistance gene module. Please note that the plasmid contains Apramycin selection, not Gentamycin.
104523LI F-G KanLI resistance gene module
104524LI F-G TetLI resistance gene module
104525LI F-G CmlLI resistance gene module
104526LI Rsc202375 UpstreamLI genomic upstream module
104527LI Rsc203337 DownstreamLI genomic downstream module
104528LI LacZLI lacZ module
104529LII destinationLII backbone vector
104530LIII 1-6 Amp RCLIII backbone vector

Antibodies from Article