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Monoubiquitination of syntaxin 3 leads to retrieval from the basolateral plasma membrane and facilitates cargo recruitment to exosomes.
Giovannone AJ, Reales E, Bhattaram P, Fraile-Ramos A, Weimbs T
Mol Biol Cell. 2017 Oct 15;28(21):2843-2853. doi: 10.1091/mbc.E17-07-0461. Epub 2017 Aug 16. PubMed Article

Plasmids from Article

ID Plasmid Purpose
99743pcDNA4/TO h-Stx3 L165A/E166A-2xMyc/HisExpresses human Stx3 locked into the open conformation with a C-term myc-myc-his tag in mammalian cells.
99746pcDNA4 h-Stx3 resistant to shRNA #304-2xMyc/HisExpresses human Stx3 with a C-term myc-myc-his that is resistant to shRNA #304 from Sigma Aldrich.

Antibodies from Article