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A Robust CRISPR Interference Gene Repression System in Pseudomonas.
Tan SZ, Reisch CR, Prather KLJ
J Bacteriol. 2018 Mar 12;200(7). pii: JB.00575-17. doi: 10.1128/JB.00575-17. Print 2018 Apr 1.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
105232pBx-Spas-sgRNA-GmPlasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Gentamycin marker for P. aeruginosa
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105233pBx-Spas-sgRNA-KanPlasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Kanamycin marker for P. putida and P. fluorescens
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105234pUC18-mini-Tn7T-Lac-dCas9 (Ptac)Tn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Ptac promoter for expression in P. putida and P. fluorescens
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105235pUC18-mini-Tn7T-Plac-dCas9 (Plac)Tn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Plac promoter for expression in P. aeruginosa
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