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pUC18-mini-Tn7T-Lac-dCas9 (Ptac)
(Plasmid #105234)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 105234 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC18
  • Backbone size w/o insert (bp) 5979
  • Total vector size (bp) 9402
  • Vector type
    Bacterial Expression, CRISPR, Synthetic Biology
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    S. pasteurianus dCas9
  • Species
    S. pasteurianus
  • Insert Size (bp)
    3423
  • Mutation
    D10A, H599A
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    S. pasteurianus dCas9 was kindly provided by Rock et al. 2017. Programmable transcriptional repression in mycobacteria using an orthogonal CRISPR interference platform. Nat Microbiol 2:16274. pUC18-mini-Tn7T-Lac was derived from Choi et al. 2006. mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa. Nat Protoc 1:153–161.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC18-mini-Tn7T-Lac-dCas9 (Ptac) was a gift from Kristala Prather (Addgene plasmid # 105234 ; http://n2t.net/addgene:105234 ; RRID:Addgene_105234)
  • For your References section:

    A Robust CRISPR Interference Gene Repression System in Pseudomonas. Tan SZ, Reisch CR, Prather KLJ. J Bacteriol. 2018 Mar 12;200(7). pii: JB.00575-17. doi: 10.1128/JB.00575-17. Print 2018 Apr 1. 10.1128/JB.00575-17 PubMed 29311279