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Predictable and precise template-free CRISPR editing of pathogenic variants.
Shen MW, Arbab M, Hsu JY, Worstell D, Culbertson SJ, Krabbe O, Cassa CA, Liu DR, Gifford DK, Sherwood RI
Nature. 2018 Nov;563(7733):646-651. doi: 10.1038/s41586-018-0686-x. Epub 2018 Nov 7.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
107186p2T-CAG-MCS-P2A-GFP-PuroRBase plasmid for cloning gene duplication alleles with eGFP reporter.
107189p2T-CAG-KKHSaCas9-BlastRConfers constitutive expression of KKH SaCas9.
107190p2T-CAG-SpCas9-BlastRConfers constitutive expression of SpCas9