Design and characterization of mutant and wildtype huntingtin proteins produced from a toolkit of scalable eukaryotic expression systems.

Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith CH
J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 10.1074/jbc.RA118.007204. Epub 2019 Mar 6. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
111723	pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q23	Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
111724	pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q73	Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
111725	pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q79	Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
111726	pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q23	Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
111727	pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q54	Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
111728	pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q73	Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
111729	pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q79	Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
111730	pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q109	Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
111731	pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q145	Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
111732	pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q15	Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
111733	pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q19	Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
111734	pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q51	Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
111735	pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q66	Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
111736	pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q85	Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
111737	pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q78	Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
111738	pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q139	Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
111741	pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q19	Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
111742	pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q24	Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
111743	pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q25	Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
111744	pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q30	Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
111745	pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q36	Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
111746	pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q42	Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
111747	pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q48	Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
111748	pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q52	Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
111749	pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q60	Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
111750	pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q66	Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
111751	pBacMam2-DiEx-LIC-N entry vector	Intermediate vector containing HTT gene, except for polyQ region. Used to clone various polyQ lengths into HTT sequence with N-term FLAG tag. Baculovirus transfer vector for insect and mammalian cells
111752	pBacMam2-DiEx-LIC-C entry vector	Intermediate vector containing HTT gene, except for polyQ region. Used to clone various polyQ lengths into HTT sequence with C-term FLAG tag. Baculovirus transfer vector for insect and mammalian cells
124060	HAP40 1-371	Baculovirus expression vector for HAP40 protein (aa 1-371) in insect cells