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Voltage-gated calcium flux mediates Escherichia coli mechanosensation.
Bruni GN, Weekley RA, Dodd BJT, Kralj JM
Proc Natl Acad Sci U S A. 2017 Aug 29;114(35):9445-9450. doi: 10.1073/pnas.1703084114. Epub 2017 Aug 14. PubMed Article

Plasmids from Article

ID Plasmid Purpose
98917pKL001Constitutively expresses the CaPR construct, the voltage indicator PROPS tethered to the calcium sensor GCaMP6f, under the Sigma 70 promoter in E. coli.
98919pKL003Constitutively expresses the voltage indicator PROPS under the Sigma 70 promoter in E. coli.
98920pKL004Constitutively expresses the calcium sensor GCaMP6f tethered to mRuby3 under the Sigma 70 promoter in E. coli.
98921pKL002pET DUET backbone with PROPS voltage indicator and GCaMP6f calcium sensor in the two MCS.

Antibodies from Article