A Transient Rise in Free Mg(2+) Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic Chromosome Condensation.
Maeshima K, Matsuda T, Shindo Y, Imamura H, Tamura S, Imai R, Kawakami S, Nagashima R, Soga T, Noji H, Oka K, Nagai T
Curr Biol. 2018 Feb 5;28(3):444-451.e6. doi: 10.1016/j.cub.2017.12.035. Epub 2018 Jan 18.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 124838 | MARIO/ pcDNA3 | mammalian expression of MARIO, a genetically-encoded fluorescent Mg2+ sensor |
| 247340 | pPB-EF1α-H2B-PA-mCherry-PGKneo | Expresses photoactivatable H2B-Cherry under EF1 promoter and can be randomly integrated by PiggyBac transposase |
| 247646 | pPB-PGKneo-EF1a-NLS-MARIO-SV40 pA | mammalian expression of NLS-MARIO, a genetically-encoded fluorescent Mg2+ sensor |