Functional Analysis of Hsh155/SF3b1 Interactions with the U2 snRNA/Branch Site Duplex.
Carrocci TJ, Paulson JC, Hoskins AA
RNA. 2018 May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 111936 | Hsh155 T178A | This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the T178A mutation |
| 111937 | Hsh155 R181A | This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the R181A mutation |
| 111938 | Hsh155 R182A | This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the R182A mutation |
| 111939 | Hsh155 R186A | This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the R186A mutation |
| 111940 | Hsh155 T178A R181A R182A R186A (N4A) | This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the T178A R181A R182A R186A mutation |
| 111941 | Hsh155 K227A | This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the K227A mutation |
| 111942 | Hsh155 Q773A | This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the Q773A mutation |
| 111943 | Hsh155 R775A | This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the R775A mutation |
| 111944 | Hsh155 Q776A | This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the Q776A mutation |
| 111945 | Hsh155 R778A | This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the R778A mutation |
| 111946 | Hsh155 Q773A R775A R776A Q778A (C4A) | This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the Q773A R775A R776A Q778A mutation |
| 111947 | Hsh155 K740A | This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the K740A mutation |
| 111948 | Hsh155 R744A | This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the R744A mutation |
| 111949 | Hsh155 N747A | This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the N747A mutation |
| 111950 | Hsh155 V783A | This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the V783A mutation |
| 111951 | Hsh155 K818A | This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the K818A mutation |
| 111952 | Hsh155 Y826A | This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the Y826A mutation |
| 111953 | Hsh155 K740R | This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the K740R mutation |
| 111954 | Hsh155 N747D | This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the N747D mutation |
| 111955 | Hsh155 V783L | This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the V783L mutation |
| 111956 | Hsh155 K818R | This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the K818R mutation |
| 111960 | Hsh155 3xFLAG | This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. |
| 111963 | Hsh155 Hs 1-5 3xFLAG | Human HEATs 1-5 have been inserted into Hsh155 3xFLAG. |
| 111965 | Hsh155 Hs 1-12 3xFLAG | Human HEATs 1-12 have been inserted into Hsh155 3xFLAG. |
| 111966 | Hsh155 Hs 1-16 3x FLAG | Human HEATs 1-16 have been inserted into Hsh155 3xFLAG. |
| 111968 | Hsh155 Hs 5-12 3xFLAG | Human HEATs 5-12 have been inserted into Hsh155 3xFLAG. |
| 111969 | Hsh155 Hs 5-16 3xFLAG | Human HEATs 5-16 have been inserted into Hsh155 3xFLAG. |
| 111971 | Hsh155 Hs 5-20 3xFLAG | Human HEATs 5-20 have been inserted into Hsh155 3xFLAG. |
| 111972 | Hsh155 Hs 12-20 3xFLAG | Human HEATs 12-20 have been inserted into Hsh155 3xFLAG. |
| 111975 | Hsh155 N4A 3xFLAG | Shuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker. It has 3xFLAG at the C-terminus. Contains the N4A mutation. |
| 111977 | Hsh155 R775A 3xFLAG | Shuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains R775A mutation. Lethal |
| 111979 | Hsh155 R778A 3xFLAG | Shuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains R778A mutation. Lethal |
| 111982 | Hsh155 K818A 3xFLAG | Shuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains K818A mutation. Lethal |
| 111987 | Hsh155 64-972 | Truncated Hsh155 (aa 64-972) |
| 111988 | Hsh155 96-972 | Truncated Hsh155 (aa 95-972) |
| 111989 | Hsh155 103-972 | Truncated Hsh155 (aa 103-972) |
| 111990 | Hsh155 157-972 | Truncated Hsh155 (aa 157-972) |
| 111991 | Hsh155 197-972 | Truncated Hsh155 (aa 197-972) |
| 111992 | Hsh155 237-972 | Truncated Hsh155 (aa 237-972) |
| 111993 | Hsh155 275-972 | Truncated Hsh155 (aa 275-972) |
| 111994 | Hsh155 310-972 | Truncated Hsh155 (aa 310-972) |
| 111995 | Hsh155 351-972 | Truncated Hsh155 (aa 351-972) |
| 111996 | Hsh155 472-972 | Truncated Hsh155 (aa 472-972) |
| 111997 | Hsh155 1-966 | Truncated Hsh155 (aa 1-966) |
| 111998 | Hsh155 1-961 | Truncated Hsh155 (aa 1-961) |
| 111999 | Hsh155 1-956 | Truncated Hsh155 (aa 1-956) |
| 112001 | Hsh155 1-951 | Truncated Hsh155 (aa 1-951) |
| 112002 | Hsh155 1-945 | Truncated Hsh155 (aa 1-945) |
| 112003 | Hsh155 1-910 | Truncated Hsh155 (aa 1-910) |