Transforming insect population control with precision guided sterile males with demonstration in flies.
Kandul NP, Liu J, Sanchez C HM, Wu SL, Marshall JM, Akbari OS
Nat Commun. 2019 Jan 8;10(1):84. doi: 10.1038/s41467-018-07964-7. PubMed Article
Kandul NP, Liu J, Sanchez C HM, Wu SL, Marshall JM, Akbari OS
Nat Commun. 2019 Jan 8;10(1):84. doi: 10.1038/s41467-018-07964-7. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 112685 | nos-Cas9.874Z1 | Express hSpCas9 under nanos promoter |
| 112686 | Ubiq-Cas9.874W | Express hSpCas9 under Ubiquitin-63E promoter |
| 112687 | vas-Cas9.874Z | Express hSpCas9 under vasa promoter |
| 112688 | gRNA[Sxl].1026B | express gRNA targeting Sxl under dU6-3 promoter |
| 112689 | gRNA[Tra].1026G | express gRNA targeting Tra under dU6-3 promoter |
| 112690 | gRNA[DsxF].1026A | express gRNA targeting DsxF under dU6-3 promoter |
| 112691 | gRNA[bTub].1026C | express gRNA targeting bTub under dU6-3 promoter |
| 112692 | gRNAs[bTub+Sxl].1046B | express two gRNA targeting bTub & Sxl under dU6-3 promoter |
| 112693 | gRNAs[bTub+Tra].1046D | express two gRNA targeting bTub & Tra under dU6-3 promoter |
| 112694 | gRNA[bTub+DsxF].1046A | express two gRNA targeting bTub & DsxF under dU6-3 promoter |
| 112695 | bTub-GFP.766A | expressed GFP in testes, under bTub promoter |