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Transforming insect population control with precision guided sterile males with demonstration in flies.
Kandul NP, Liu J, Sanchez C HM, Wu SL, Marshall JM, Akbari OS
Nat Commun. 2019 Jan 8;10(1):84. doi: 10.1038/s41467-018-07964-7.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
112685nos-Cas9.874Z1Express hSpCas9 under nanos promoter
112686Ubiq-Cas9.874WExpress hSpCas9 under Ubiquitin-63E promoter
112687vas-Cas9.874ZExpress hSpCas9 under vasa promoter
112688gRNA[Sxl].1026Bexpress gRNA targeting Sxl under dU6-3 promoter
112689gRNA[Tra].1026Gexpress gRNA targeting Tra under dU6-3 promoter
112690gRNA[DsxF].1026Aexpress gRNA targeting DsxF under dU6-3 promoter
112691gRNA[bTub].1026Cexpress gRNA targeting bTub under dU6-3 promoter
112692gRNAs[bTub+Sxl].1046Bexpress two gRNA targeting bTub & Sxl under dU6-3 promoter
112693gRNAs[bTub+Tra].1046Dexpress two gRNA targeting bTub & Tra under dU6-3 promoter
112694gRNA[bTub+DsxF].1046Aexpress two gRNA targeting bTub & DsxF under dU6-3 promoter
112695bTub-GFP.766Aexpressed GFP in testes, under bTub promoter

Antibodies from Article