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Covalent linkage of the DNA repair template to the CRISPR-Cas9 nuclease enhances homology-directed repair.
Savic N, Ringnalda FC, Lindsay H, Berk C, Bargsten K, Li Y, Neri D, Robinson MD, Ciaudo C, Hall J, Jinek M, Schwank G
Elife. 2018 May 29;7. pii: 33761. doi: 10.7554/eLife.33761.
PubMed

Plasmids from Article

ID Plasmid Purpose  
113716pNS19-LV-mutRFP-2A-GFPLentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)
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113717pNS20-SpCas9-SNAPBacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9
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113718pNS38-SadCas9-SNAPBacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9
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