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(Plasmid #113718)


Item Catalog # Description Quantity Price (USD)
Plasmid 113718 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    pET His6 MBP N10 TEV LIC cloning vector (2C-T)
  • Backbone manufacturer
    Scott Gradia
  • Backbone size w/o insert (bp) 5938
  • Total vector size (bp) 9758
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
  • Alt name
  • Species
    Staphylococcus aureus
  • Insert Size (bp)
  • Mutation
    D10A, N580A
  • GenBank ID
  • Tags / Fusion Proteins
    • His6-MBP-TEV (N terminal on backbone)
    • HA-2xNLS-SNAP-NLS (C terminal on insert)

Resource Information

Depositor Comments

Cloning of pNS38-SadCas9-SNAP: pAD-SaCas9-GFP was generated by replacing the SpCas9 coding sequence in pMJ922 with SaCas9 sequence using Gibson cloning (Keppler et al., 2003). QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies) was used to remove the stop codon and to introduce the D10A and N580A mutations into the SaCas9 (SadCas9) gene. Subsequently, GFP was cut out using BamHI and KpnI, and replaced by a SNAP-tag-NLS gBlock (Integrated DNA Technologies) using In-Fusion HD Cloning Plus CE (Takara).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNS38-SadCas9-SNAP was a gift from Gerald Schwank (Addgene plasmid # 113718 ; ; RRID:Addgene_113718)
  • For your References section:

    Covalent linkage of the DNA repair template to the CRISPR-Cas9 nuclease enhances homology-directed repair. Savic N, Ringnalda FC, Lindsay H, Berk C, Bargsten K, Li Y, Neri D, Robinson MD, Ciaudo C, Hall J, Jinek M, Schwank G. Elife. 2018 May 29;7. pii: 33761. doi: 10.7554/eLife.33761. 33761 [pii] PubMed 29809142