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pNS19-LV-mutRFP-2A-GFP
(Plasmid #113716)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 113716 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Duet011
  • Backbone size w/o insert (bp) 8339
  • Total vector size (bp) 11062
  • Vector type
    Mammalian Expression, Lentiviral ; Reporter system
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mutRFP-2A-GFP-IRES-Puro
  • Alt name
    eGFP
  • Alt name
    mutated turbo RFP
  • Alt name
    Puromycin resistant gene
  • Insert Size (bp)
    2723
  • Mutation
    Y64L (mutRFP)

Resource Information

Depositor Comments

Cloning of pNS19-LV-mutRFP-2A-GFP: pEGIP (addgene plasmid #26777) was mutagenized using QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies) to destroy the start codon of eGFP. Next the vector was linearized with BamHI and In-Fusion HD Cloning Plus CE (Takara) was used to insert the mutRFP-2A gBlocks Gene Fragment (Integrated DNA Technologies).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNS19-LV-mutRFP-2A-GFP was a gift from Gerald Schwank (Addgene plasmid # 113716 ; http://n2t.net/addgene:113716 ; RRID:Addgene_113716)
  • For your References section:

    Covalent linkage of the DNA repair template to the CRISPR-Cas9 nuclease enhances homology-directed repair. Savic N, Ringnalda FC, Lindsay H, Berk C, Bargsten K, Li Y, Neri D, Robinson MD, Ciaudo C, Hall J, Jinek M, Schwank G. Elife. 2018 May 29;7. pii: 33761. doi: 10.7554/eLife.33761. 33761 [pii] PubMed 29809142