Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #113716)


Item Catalog # Description Quantity Price (USD)
Plasmid 113716 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 8339
  • Total vector size (bp) 11062
  • Vector type
    Mammalian Expression, Lentiviral ; Reporter system
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Alt name
    mutated turbo RFP
  • Alt name
    Puromycin resistant gene
  • Insert Size (bp)
  • Mutation
    Y64L (mutRFP)

Resource Information

Depositor Comments

Cloning of pNS19-LV-mutRFP-2A-GFP: pEGIP (addgene plasmid #26777) was mutagenized using QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies) to destroy the start codon of eGFP. Next the vector was linearized with BamHI and In-Fusion HD Cloning Plus CE (Takara) was used to insert the mutRFP-2A gBlocks Gene Fragment (Integrated DNA Technologies).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNS19-LV-mutRFP-2A-GFP was a gift from Gerald Schwank (Addgene plasmid # 113716 ; ; RRID:Addgene_113716)
  • For your References section:

    Covalent linkage of the DNA repair template to the CRISPR-Cas9 nuclease enhances homology-directed repair. Savic N, Ringnalda FC, Lindsay H, Berk C, Bargsten K, Li Y, Neri D, Robinson MD, Ciaudo C, Hall J, Jinek M, Schwank G. Elife. 2018 May 29;7. pii: 33761. doi: 10.7554/eLife.33761. 33761 [pii] PubMed 29809142