Poly(Beta-Amino Ester) Nanoparticles Enable Nonviral Delivery of CRISPR-Cas9 Plasmids for Gene Knockout and Gene Deletion.
Rui Y, Varanasi M, Mendes S, Yamagata HM, Wilson DR, Green JJ
Mol Ther Nucleic Acids. 2020 Apr 21;20:661-672. doi: 10.1016/j.omtn.2020.04.005. PubMed Article
Rui Y, Varanasi M, Mendes S, Yamagata HM, Wilson DR, Green JJ
Mol Ther Nucleic Acids. 2020 Apr 21;20:661-672. doi: 10.1016/j.omtn.2020.04.005. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 113966 | sg1 | Single short guide RNA targeting GTATAGCATACATTATACG |
| 113967 | sg2 | Single short guide RNA targeting TACCACATTTGTAGAGGTT |
| 113968 | sg3 | Single short guide RNA targeting CAATGTATCTTATCATGTC |
| 113969 | sg1+sg2+sg3 | Triple short guide RNA targeting GTATAGCATACATTATACG, TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTC |
| 113970 | sg2+sg3 | Double short guide RNA targeting TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTC |
| 113972 | sgiRFP1 | Single short guide RNA targeting GATCGAGTTCGAGCCTGCGG in iRFP670 sequence |
| 113973 | sgiRFP2 | Single short guide RNA targeting GCGCGTTCTTTGGACGCGA in iRFP670 sequence |
| 113974 | sgiRFP3 | Single short guide RNA targeting CGTGATGTTGTACCGCTTC in iRFP670 sequence |
| 127197 | pGTRm | pGTRm is the template plasmid for PCR & golden gate assembly driven generation of polycistronic tRNA-gRNA sequences |
| 127198 | PTG | Single template U6 promoter minimal size plasmid |
| 127199 | pPTG-GG-sg2+sg3 | Plasmid expressing sgRNA2 (TACCACATTTGTAGAGGTT) & sgRNA3 (CAATGTATCTTATCATGTC) as polycistronic tRNA-gRNA from single U6 promoter |