Characterization of Polyketide Synthase Machinery from the pks Island Facilitates Isolation of a Candidate Precolibactin.
Zha L, Wilson MR, Brotherton CA, Balskus EP
ACS Chem Biol. 2016 May 20;11(5):1287-95. doi: 10.1021/acschembio.6b00014. Epub 2016 Mar 1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 114145 | pET28a-clbC | Expresses ClbC-nHis |
| 114146 | pET29b-clbD | Expresses ClbD-cHis |
| 114147 | pET29b-clbE | Expresses ClbE-cHis |
| 114148 | pET29b-clbF | Expresses ClbF-cHis |
| 114149 | pET28a-clbH-A1 | Expresses ClbH-A1 |
| 114150 | pET29b-clbG | Expresses ClbG |
| 114151 | pET29b-clbI | Expresses ClbI |
| 114152 | pET28a-clbK | Expresses ClbK |
| 114153 | pET29b-clbO | Expresses ClbO |
| 114154 | pET29b-clbBpks | Expresses the PKS module of ClbB |