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CRISPR-Cas12a-Assisted Recombineering in Bacteria.
Yan MY, Yan HQ, Ren GX, Zhao JP, Guo XP, Sun YC
Appl Environ Microbiol. 2017 Aug 17;83(17). pii: AEM.00947-17. doi: 10.1128/AEM.00947-17. Print 2017 Sep 1.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
158706pJV53-Cas12aCRISPR system used for genome editing in Mycobacterium smegmatis, expresses codon-optimized FnCpf1 and the recombination proteins gp60 and gp61
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158707pMV261-Cas12aexpresses codon-optimized FnCpf1
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158708pCR-HygFor cloning and constitutive expression of crRNAs in mycobacterium
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158709pCR-ZeoFor cloning and constitutive expression of crRNAs in mycobacterium
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158711pAC-crRNA-CmPlasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA
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158712pAC-crRNA-KmPlasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA
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