Visualization and Quantification of Cell-to-cell Movement of Proteins in Nicotiana benthamiana.
Blekemolen, M. C., Tark-Dame, M. and Takken, F. L.
Bio-protocol 8(24): e3114.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 118011 | Avr2-GFP pZK538 | expresses Avr2-GFP (without signal peptide) and mCherry-HDEL. Used for visualization of cell-to-cell movement of Avr2. Avr2 can be replaced with gene of interest via HiFi DNA assembly cloning (NEB) . |
| 118012 | NSm-GFP pZK538 | expresses NSm-GFP and mCherry-HDEL. Used for visualization of cell-to-cell movement of NSm. NSm can be replaced with gene of interest via HiFi DNA assembly cloning (NEB). |
| 118013 | Six5 pGWB402 | expresses Six5 (without signal peptide). Used in combination with Avr2-GFP pZK538(#118011) for visualization of cell-to-cell movement of Avr2 |