A Highly Sensitive A-Kinase Activity Reporter for Imaging Neuromodulatory Events in Awake Mice.
Ma L, Jongbloets BC, Xiong WH, Melander JB, Qin M, Lameyer TJ, Harrison MF, Zemelman BV, Mao T, Zhong H
Neuron. 2018 Aug 22;99(4):665-679.e5. doi: 10.1016/j.neuron.2018.07.020. Epub 2018 Aug 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 119912 | pCDNA3_AKAR5Δ | Mammalian expression of the AKAR5Δ sensor with N-terminal His tag for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM) |
| 119913 | pRK5_tAKARα | Mammalian expression of the tAKARα sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM) |
| 119914 | pRK5_tAKARβ | Mammalian expression of the tAKARβ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM) |
| 119915 | pRK5_tAKARγ | Mammalian expression of the tAKARγ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM) |
| 119916 | pRK5_tAKARδ | Mammalian expression of the tAKARδ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM) |
| 119917 | pRK5_tAKARε | Mammalian expression of the tAKARε sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM) |
| 119918 | pRK5_tAKARζ | Mammalian expression of the tAKARζ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM) |
| 119919 | pRK5_tAKAR4α | Mammalian expression of the tAKAR4α sensor for detecting PKA activity using ratiometric imaging |
| 119920 | pRK5_tAKARα-T391A | Mammalian expression of the tAKARα sensor with T391A mutation |
| 119921 | pAAV-hSYNCMV tAKARα | AAV construct with tAKARα being expressed under the human synapsin promotor with WPRE enhancer. |