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A xylose-inducible expression system and a CRISPRi-plasmid for targeted knock-down of gene expression in Clostridioides difficile.
Muh U, Pannullo AG, Weiss DS, Ellermeier CD
J Bacteriol. 2019 Feb 11. pii: JB.00711-18. doi: 10.1128/JB.00711-18. PubMed Article

Plasmids from Article

ID Plasmid Purpose  
120799pAP114E. coli-C. difficile shuttle vector for xylose-inducible expression in C. difficile; Pxyl driving expression of red fluorescent protein (mCherryOpt)
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120803pIA33E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp
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Recombinant Antibodies from Article