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(Plasmid #120803)


Item Catalog # Description Quantity Price (USD)
Plasmid 120803 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 6400
  • Total vector size (bp) 12940
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Low copy in C. difficile following conjugation and selected with thiamphenicol
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    deactivated nuclease dCas9, codon-optimized for C. difficile
  • Alt name
  • Species
    S. pyogenes
  • Insert Size (bp)
  • Mutation
    Codon optimized for C. difficile; H840A and D10A to deactivate nuclease
  • Promoter Pxyl

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    single guide RNA targeting red fluorescent protein
  • Alt name
  • Species
    Synthetic; S. pyogenes
  • Insert Size (bp)
  • Mutation
    Base-pairing region targets red fluorescent protein (rfp)
  • Promoter Pgdh

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIA33 was a gift from Craig Ellermeier & David S. Weiss (Addgene plasmid # 120803 ; ; RRID:Addgene_120803)
  • For your References section:

    A xylose-inducible expression system and a CRISPRi-plasmid for targeted knock-down of gene expression in Clostridioides difficile. Muh U, Pannullo AG, Weiss DS, Ellermeier CD. J Bacteriol. 2019 Feb 11. pii: JB.00711-18. doi: 10.1128/JB.00711-18. 10.1128/JB.00711-18 PubMed 30745377